Increased yield of tri- and tetranucleotide heterospecific microsatellites from unenriched small-insert libraries.
نویسندگان
چکیده
منابع مشابه
Distribution of Polymorphic and Non-Polymorphic Microsatellite Repeats in Xenopus tropicalis
The results of our bioinformatics analysis have found over 91,000 di-, tri-, and tetranucleotide microsatellites in our survey of 25% of the X. tropicalis genome, suggesting there may be over 360,000 within the entire genome. Within the X. tropicalis genome, dinucleotide (78.7%) microsatellites vastly out numbered tri- and tetranucleotide microsatellites. Similarly, AT-rich repeats are overwhel...
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Polymerase chain reaction primer pairs for a total of 25 nuclear-encoded microsatellites (loci) were developed from genomic DNA libraries of lane snapper ( Lutjanus synagris ), mutton snapper ( Lutjanus analis ), and yellowtail snapper ( Ocyurus chrysurus ). The microsatellites include 24 perfect (21 dinucleotide and three trinucleotide) and one imperfect (combination tetranucleotide/tetranucle...
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Microsatellite polymorphisms are widely being employed as a major tool in genome analysis. Analysis of length polymorphisms in the PCR depends on the elucidation of the sequence of their flanking regions. Different methods have been developed for the efficient characterization of the flanking sequences. Small insert genomic libraries directly suitable for sequencing of the entire insert and hig...
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Thirteen polymorphic tri- and tetranucleotide microsatellite markers are reported for the exploited marine dhufish (Glaucosoma hebracium) from southwestern Australia. Variation was assessed among 48 individuals collected from a single locality. Most loci had 2-5 alleles, although one had more than 20 alleles, with corresponding observed heterozygosities of 0.02-0.81. These loci were also polymo...
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A large insert genomic library from eastern white pine (Pinus strobus) was probed for the microsatellite motifs (AC)n and (AG)n, all 10 trinucleotide motifs, and 22 of the 33 possible tetranucleotide motifs. For comparison with a species from a different subgenus, a loblolly pine (Pinus taeda) genomic library was also probed with the same set of di- and tri-nucleotide repeats and 11 of the tetr...
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ورودعنوان ژورنال:
- BioTechniques
دوره 24 1 شماره
صفحات -
تاریخ انتشار 1998